|
KMID : 1161420150180050542
|
|
Journal of Medicinal Food
2015 Volume.18 No. 5 p.542 ~ p.548
|
|
|
Effect of Fermented Red Ginseng Extract Enriched in Ginsenoside Rg3 on the Differentiation and Mineralization of Preosteoblastic MC3T3-E1 Cells
|
|
Siddiqi Muhammad Zubair
Siddiqi Muhammad Hanif
Kim Yeon-Ju
Jin Yan
Huq Md. Amdadul
Yang Deok-Chun
|
|
|
Abstract
|
|
|
|
In this study, red ginseng extract (RGE) was converted into high-content minor ginsenosides by fermenting with Bgp1 enzymes at 37°C for 5 days. Compared to the RGE, the minor ginsenoside contents were increased in fermented red ginseng extract (FRGE). Moreover, the amount of minor ginsenosides such as Rh1 (11%) and Rg2 (16%) was slightly augmented, while the level of Rg3 (33%) was significantly increased after bioconversion. Furthermore, we also examined and compared the effect of RGE and FRGE on the differentiation and mineralization of preosteoblastic MC3T3-E1 cells. Similarly, the level of mRNA expression of intracellular alkaline phosphatase (ALP) activity, type-1 collagen (Col-I) was also increased. Based on the comparison, it is clear that the FRGE has improved effects on bone formation and differentiation of preosteoblastic MC3T3-E1 cells.
|
|
|
KEYWORD
|
|
|
β-glycosidase, bone formation, differentiation, mineralization, osteoblast, Panax ginseng
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|